pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
(Plasmid #31482)

• Depositing Lab: Andrew Scharenberg
• Publication: Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.

Backbone

• Vector backbone: pCVL
• Backbone size w/o insert (bp): 5000
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Traffic Light Reporter 1.1 (Sce Target) EF Puro
• Insert Size (bp): 2803
• Mutation: mCherry has M9S and M16L mutations. This is what the ".1" means.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 24 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry