pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
(Plasmid
#31478)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCVL
- Backbone size w/o insert (bp) 5000
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSFFV HA NLS Y2 K227M T2A BFP
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Insert Size (bp)2010
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MutationF13Y, S111Y, K227M = Y2 nickase "reo" (restriction endonuclease optimized) denotes that the coding sequence has had silent restriction sites placed into it to aid in enzyme redesign.
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Tags
/ Fusion Proteins
- HA
- NLS
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP was a gift from Andrew Scharenberg (Addgene plasmid # 31478 ; http://n2t.net/addgene:31478 ; RRID:Addgene_31478) -
For your References section:
Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461
Map uploaded by the depositor.
