pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
(Plasmid #31477)

• Depositing Lab: Andrew Scharenberg
• Publication: Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.

Backbone

• Vector backbone: pCVL
• Backbone size w/o insert (bp): 5000
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SFFV HA NLS Y2 T2A mTagBFP
• Insert Size (bp): 2010
• Mutation: F13Y + S111Y on I-AniI = Y2 "reo" (restriction endonuclease optimized) denotes that the coding sequence has had silent restriction sites placed into it to aid in enzyme redesign.
• Tags / Fusion Proteins:
  • HA
  • NLS

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry