Skip to main content
Addgene

pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
(Plasmid #31476)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCVL
  • Backbone size w/o insert (bp) 5092
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SFFV d14 GFP donor EF1s I-SceI
  • Insert Size (bp)
    2173
  • Mutation
    GFP has 14 amino acids deleted from c terminus
  • Tags / Fusion Proteins
    • HA
    • NLS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt) was a gift from Andrew Scharenberg (Addgene plasmid # 31476 ; http://n2t.net/addgene:31476 ; RRID:Addgene_31476)

  • For your References section:

    Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461
Related items:
Commonly requested with: