GW1-pHRed
(Plasmid #31473)

• Purpose: genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima
• Depositing Lab: Gary Yellen
• Publication: Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034-7.

Backbone

• Vector backbone: GW1
• Backbone manufacturer: British Biotechnology
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pHRed
• Species: Synthetic
• Insert Size (bp): 710

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CACAGCAATCGATCCATG

Resource Information

• Supplemental Documents:
  • Supplemental Information from the Yellen lab
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry