pRsetB-pHRed
(Plasmid #31472)

• Depositing Lab: Gary Yellen
• Publication: Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034-7.

Backbone

• Vector backbone: pRsetB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2760
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pHRed
• Species: Synthetic
• Insert Size (bp): 750
• Tag / Fusion Protein:
  • 7xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Supplemental Information from the Yellen lab
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry