pTRETightBI-RY-SLC6A1
(Plasmid #31471)

• Depositing Lab: Phil Sharp
• Publication: Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905.

Backbone

• Vector backbone: pTRETightBI
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 2850
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10
• Growth instructions: Grow TopTen cells at 30C instead of 37C to reduce risk of recombination at the SV40 pA sites.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eYFP and mCherry
• Species: fluorescent proteins
• Insert Size (bp): 740
• Tag / Fusion Protein:
  • NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, BamHI (not destroyed)
• 3′ cloning site: NdeI, ClaI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mCherry fused 3'UTR composed of SLC6A1, which contains three binding sites for the neuronal miRNA miR-218