pINT-PuroDDEYFP
(Plasmid
#31442)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCSGFP
- Backbone size w/o insert (bp) 3500
-
Vector typeMammalian Expression ; phiC31/attB
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameenhanced yellow fluorescent protein
-
Alt nameEYFP
-
Insert Size (bp)2000
-
Tag
/ Fusion Protein
- FKBP L106P (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Integration vector with attB site for phiC31 mediated integration of EYFP into attP site using puromycin selection. EYFP linked to destabilization domain (FKBP L106P) allowing shield inducibility.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pINT-PuroDDEYFP was a gift from Gerhart Ryffel (Addgene plasmid # 31442 ; http://n2t.net/addgene:31442 ; RRID:Addgene_31442) -
For your References section:
Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU. BMC Res Notes. 2011 Oct 18;4:420. 10.1186/1756-0500-4-420 PubMed 22008483
Map uploaded by the depositor.
