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pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins
(Plasmid #31440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSGFP2
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression, Cre/Lox ; Flp/FRT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HNF1B
  • Alt name
    LFB3
  • Alt name
    vHNF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4612
  • Mutation
    A263insGG
  • GenBank ID
    NM_001165923
  • Entrez Gene
    HNF1B (a.k.a. ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cre and FLP activator construct: from blue fluorescence to expression of HNF1B mutant

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins was a gift from Gerhart Ryffel (Addgene plasmid # 31440 ; http://n2t.net/addgene:31440 ; RRID:Addgene_31440)

  • For your References section:

    Heat-shock mediated overexpression of HNF1beta mutations has differential effects on gene expression in the Xenopus pronephric kidney. Sauert K, Kahnert S, Roose M, Gull M, Brandli AW, Ryffel GU, Waldner C. PLoS One. 2012;7(3):e33522. Epub 2012 Mar 15. 10.1371/journal.pone.0033522 PubMed 22438943
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