pLCMV:ECFP(loxP)(FRT)HNF1B
(Plasmid #31439)

• Depositing Lab: Gerhart Ryffel
• Publication: Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Mar 15.

Backbone

• Vector backbone: pCSGFP2
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression, Cre/Lox ; Flp/FRT

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HNF1B
• Alt name: LFB3
• Alt name: vHNF1
• Species: H. sapiens (human)
• Insert Size (bp): 4612
• GenBank ID: NM_001165923
• Entrez Gene: HNF1B (a.k.a. ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre and FLP activator construct: from blue fluorescence to expression of wild type HNF1B

Please note that there are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. These discrepancies should not affect the function of the plasmid.