Ube2e3
(Plasmid
#31415)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACT2
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 8100
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbiquitin Conjugating Enzyme
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Alt nameUbcH9, UbcM2, UBCE4
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SpeciesH. sapiens (human)
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Insert Size (bp)400
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Entrez GeneUBE2E3 (a.k.a. UBCH9, UbcM2)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer GAL4 AD (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ube2e3 was a gift from Rachel Klevit (Addgene plasmid # 31415 ; http://n2t.net/addgene:31415 ; RRID:Addgene_31415) -
For your References section:
E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. Christensen DE, Brzovic PS, Klevit RE. Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. 10.1038/nsmb1295 PubMed 17873885