pCHNFI-B2
(Plasmid #31405)

• Depositing Lab: Richard Gronostajski
• Publication: Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-46.

Backbone

• Vector backbone: pCMVbeta
• Backbone manufacturer: MacGregor and Caskey
• Backbone size w/o insert (bp): 3771
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nuclear Factor IB isoform 2
• Alt name: NFIB2
• Species: M. musculus (mouse)
• Insert Size (bp): 1570
• Mutation: Not the same as reference seq. See sequence for missing exons
• GenBank ID: NM_001113209
• Entrez Gene: Nfib (a.k.a. 6720429L07Rik, CTF, E030026I10Rik, NF-I/B, NF1-B, NFI-B)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence NP_001106680.1, the NFIB2 insert in this plasmid ends at amino acid 415.