pCHNFI-A4
(Plasmid
#31404)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMVbeta
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNuclear Factor IA isoform 4
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Alt nameNFIA4
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Alt nameNFIA
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SpeciesM. musculus (mouse)
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Insert Size (bp)2021
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GenBank IDCAM26612
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Entrez GeneNfia (a.k.a. 1110047K16Rik, 9430022M17Rik, CTF, NF1-A, NF1A)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCHNFI-A4 was a gift from Richard Gronostajski (Addgene plasmid # 31404) -
For your References section:
Nuclear factor I (NFI) isoforms differentially activate simple versus complex NFI-responsive promoters. Chaudhry AZ, Vitullo AD, Gronostajski RM. J Biol Chem. 1998 Jul 17. 273(29):18538-46. 10.1074/jbc.273.29.18538 PubMed 9660824