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Addgene

pEG3a
(Plasmid #31398)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31398 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAD33
  • Backbone size w/o insert (bp) 5352
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Any E. coli strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zapB
  • Alt name
    yiiU
  • Species
    E. coli
  • Insert Size (bp)
    973
  • GenBank ID
    ECK3920
  • Entrez Gene
    zapB (a.k.a. ECK3920, JW3899, yiiU)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer Not commercial
  • 3′ sequencing primer Not commercial
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG3a was a gift from Kenn Gerdes (Addgene plasmid # 31398 ; http://n2t.net/addgene:31398 ; RRID:Addgene_31398)
  • For your References section:

    Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division. Ebersbach G, Galli E, Moller-Jensen J, Loewe J, Gerdes K. Mol Microbiol. 2008 May . 68(3):720-35. 10.1111/j.1365-2958.2008.06190.x PubMed 18394147