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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31391 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCV
- Backbone size w/o insert (bp) 6700
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Vector typeMammalian Expression, Retroviral
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Selectable markersnone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameB-cell lymphoma 6
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Alt nameBCL6
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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GenBank IDNM_001706
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Entrez GeneBCL6 (a.k.a. BCL5, BCL6A, LAZ3, ZBTB27, ZNF51)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-BCL6-IRES-GFP was a gift from Hilda Ye (Addgene plasmid # 31391 ; http://n2t.net/addgene:31391 ; RRID:Addgene_31391) -
For your References section:
BCL-6 negatively regulates macrophage proliferation by suppressing autocrine IL-6 production. Yu RY, Wang X, Pixley FJ, Yu JJ, Dent AL, Broxmeyer HE, Stanley ER, Ye BH. Blood. 2005 Feb 15. 105(4):1777-84. 10.1182/blood-2004-08-3171 PubMed 15507530