pINS-Neo
(Plasmid
#31390)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUNI10
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Backbone manufacturerStephen Elledge
- Backbone size w/o insert (bp) 1955
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Vector typeCre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW23474
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Growth instructionsBW23474 (pir::116), LB 37°C
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameaph
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Alt nameaminoglycoside phosphotransferase
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SpeciesTn5
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Insert Size (bp)1474
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPromega, Madison, WI, USA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINS-Neo was a gift from Yegor Vassetzky (Addgene plasmid # 31390 ; http://n2t.net/addgene:31390 ; RRID:Addgene_31390) -
For your References section:
A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination. Dmitriev PV, Vassetzky YS. BMC Res Notes. 2008 . 1():135. 10.1186/1756-0500-1-135 PubMed 19105832
Map uploaded by the depositor.
