pINS-Blast
(Plasmid #31388)

• Depositing Lab: Yegor Vassetzky
• Publication: Dmitriev et al BMC Res Notes. 2008 . 1():135.

Backbone

• Vector backbone: pUNI10
• Backbone manufacturer: Stephen Elledge
• Backbone size w/o insert (bp): 1843
• Vector type: Cre/Lox
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BW23474 (pir::116)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: bsd
• Alt name: blasticidin S deaminase
• Species: Aspergillus terreus
• Insert Size (bp): 1150

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmnI/EcoRV (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: sequence

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Invitrogen, Carlsbad, CA, USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

6nt mismatch compared to full plasmid sequence, does not affect the functionality of the plasmid.