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hPDI1-18-508_C400S-pFLAG-CMV1
(Plasmid #31384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAG_CMV1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4732
  • Total vector size (bp) 6188
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPDI1
  • Alt name
    prolyl 4-hydroxylase, beta polypeptide
  • Alt name
    PDIA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1514
  • Mutation
    C400S
  • Entrez Gene
    P4HB (a.k.a. CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB)
  • Tag / Fusion Protein
    • FLAG M1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer HGH-polyA reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPDI1-18-508_C400S-pFLAG-CMV1 was a gift from David Ron (Addgene plasmid # 31384 ; http://n2t.net/addgene:31384 ; RRID:Addgene_31384)

  • For your References section:

    Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin. Zito E, Melo EP, Yang Y, Wahlander Å, Neubert TA, Ron D. Mol Cell. 2010 Dec 10. 40(5):787-97. 10.1016/j.molcel.2010.11.010 PubMed 21145486
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