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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAdEasy
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 33000
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namertTA-IRES-BirA
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Insert Size (bp)33000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAd:CMV-rtTA-IRES-birA was a gift from William Pu (Addgene plasmid # 31375 ; http://n2t.net/addgene:31375 ; RRID:Addgene_31375) -
For your References section:
Genome-wide location analysis by pull down of in vivo biotinylated transcription factors. He A, Pu WT. Curr Protoc Mol Biol. 2010 Oct . Chapter 21():Unit 21.20. 10.1002/0471142727.mb2120s92 PubMed 20890903
