pP2BA
(Plasmid
#31369)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE-puro
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePer2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3797
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Entrez GenePer2 (a.k.a. mKIAA0347, mPer2)
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Tag
/ Fusion Protein
- flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pP2BA was a gift from Aziz Sancar (Addgene plasmid # 31369 ; http://n2t.net/addgene:31369 ; RRID:Addgene_31369) -
For your References section:
Biochemical analysis of the canonical model for the mammalian circadian clock. Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A. J Biol Chem. 2011 May 27. ():. 10.1074/jbc.M111.254680 PubMed 21613214