pCKPC4
(Plasmid #31366)

• Depositing Lab: Aziz Sancar
• Publication: Ye et al J Biol Chem. 2011 May 27. ():.

Backbone

• Vector backbone: pcDNA4a
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Clock
• Species: M. musculus (mouse)
• Insert Size (bp): 2613
• Entrez Gene: Clock (a.k.a. 5330400M04Rik, KAT13D)
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry