p6351 MSCV-CMV-CMV-Flag-HA-NSD3
(Plasmid #31357)

• Depositing Lab: Peter Howley
• Publication: Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52.

Backbone

• Vector backbone: pDEST-iTAP
• Backbone size w/o insert (bp): 8700
• Modifications to backbone: Note that this is a Gateway cloning vector. The insert replaces a large (approx. 1.7kb) cassette between the attR sites. Please take this into consideration when designing diagnostic digests.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NSD3
• Species: H. sapiens (human)
• Entrez Gene: NSD3 (a.k.a. KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328)
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMVf
• 3′ sequencing primer: MSCV-2

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry