p6345 MSCV-CMV-Flag-HA-Brd4 1-722
(Plasmid #31352)

• Depositing Lab: Peter Howley
• Publication: Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52.

Backbone

• Vector backbone: MSCV-CMV
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Brd4 1-722
• Species: H. sapiens (human)
• Insert Size (bp): 4100
• Mutation: *see comments
• Entrez Gene: BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVf
• 3′ sequencing primer: MSCV-2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*The MSCV-CMV-Brd4-1-722 was made from pENTR-Brd4 1-722. But the gateway LR recombination reaction seems to select for the internal aa 719 position DNA sequence, and the insert for this plasmid actually only codes up to amino acid 719. The name has been kept for consistency.