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Addgene

pSKB2-TRexR90D
(Plasmid #31350)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSKB2 (pET28b derived)
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thermus aquaticus Redox Sensing Repressor (R90D)
  • Alt name
    Rex
  • Alt name
    p25
  • Alt name
    T-Rex
  • Species
    Thermus Aquaticus
  • Insert Size (bp)
    1139
  • Mutation
    changed Arginine 90 to Aspartic Acid
  • GenBank ID
    AF061257
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKB2-TRexR90D was a gift from Clara Kielkopf (Addgene plasmid # 31350 ; http://n2t.net/addgene:31350 ; RRID:Addgene_31350)

  • For your References section:

    Structural basis for NADH/NAD+ redox sensing by a Rex family repressor. McLaughlin KJ, Strain-Damerell CM, Xie K, Brekasis D, Soares AS, Paget MS, Kielkopf CL. Mol Cell. 2010 May 28. 38(4):563-75. 10.1016/j.molcel.2010.05.006 PubMed 20513431
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