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Addgene

pET-MRPL15
(Plasmid #31345)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression
  • Selectable markers
    chloramphenicol

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    IN E. coli BL21(DE3) RIL. the RIL plasmid confers chloramphenicol resistance
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitochondrial ribosomal protein L15 mature form
  • Alt name
    MRPL15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    741
  • Mutation
    Has the amino acids in the predicted mature form of MRPL15 after removal of the putative mitochondrial import signal Amino acids 49-296
  • Entrez Gene
    MRPL15 (a.k.a. HSPC145, L15mt, MRP-L15, MRP-L7, RPML7, uL15m)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer vector
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATCC MGC-4898

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-MRPL15 was a gift from Linda Spremulli (Addgene plasmid # 31345 ; http://n2t.net/addgene:31345 ; RRID:Addgene_31345)

  • For your References section:

    The effect of mutated mitochondrial ribosomal proteins S16 and S22 on the assembly of the small and large ribosomal subunits in human mitochondria. Emdadul Haque M, Grasso D, Miller C, Spremulli LL, Saada A. Mitochondrion. 2008 Jun . 8(3):254-61. 10.1016/j.mito.2008.04.004 PubMed 18539099
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