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Addgene

pET21-MRPL13
(Plasmid #31344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression
  • Selectable markers
    chloramphenicol

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli BL21 (DE3) RIL has chloramphenicol resistance on RIL plasmid
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitochondrial ribosomal protein L13 mature form
  • Alt name
    MRPL13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    414
  • Mutation
    Mature form after removal of the predicted mitochondrial import signal Amino acids 40-178
  • Entrez Gene
    MRPL13 (a.k.a. L13, L13A, L13mt, RPL13, RPML13)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer vector
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATCC MGC-33899
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-MRPL13 was a gift from Linda Spremulli (Addgene plasmid # 31344 ; http://n2t.net/addgene:31344 ; RRID:Addgene_31344)

  • For your References section:

    The effect of mutated mitochondrial ribosomal proteins S16 and S22 on the assembly of the small and large ribosomal subunits in human mitochondria. Emdadul Haque M, Grasso D, Miller C, Spremulli LL, Saada A. Mitochondrion. 2008 Jun . 8(3):254-61. 10.1016/j.mito.2008.04.004 PubMed 18539099
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