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Addgene

pcDNA3-LacR-TopBP1
(Plasmid #31317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TopBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4600
  • Entrez Gene
    TOPBP1 (a.k.a. Dpb11, TOP2BP1)
  • Tags / Fusion Proteins
    • LacR (N terminal on insert)
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-LacR-TopBP1 was a gift from Aziz Sancar (Addgene plasmid # 31317 ; http://n2t.net/addgene:31317 ; RRID:Addgene_31317)

  • For your References section:

    Tethering DNA Damage Checkpoint Mediator Proteins Topoisomerase II{beta}-binding Protein 1 (TopBP1) and Claspin to DNA Activates Ataxia-Telangiectasia Mutated and RAD3-related (ATR) Phosphorylation of Checkpoint Kinase 1 (Chk1). Lindsey-Boltz LA, Sancar A. J Biol Chem. 2011 Jun 3. 286(22):19229-36. 10.1074/jbc.M111.237958 PubMed 21502314
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