pcDNA5-FRT/TO-LacR-TopBP1
(Plasmid #31313)

• Depositing Lab: Aziz Sancar
• Publication: Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-36.

Backbone

• Vector backbone: pcDNA5-FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5137
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TopBP1
• Species: H. sapiens (human)
• Insert Size (bp): 4600
• Entrez Gene: TOPBP1 (a.k.a. Dpb11, TOP2BP1)
• Tags / Fusion Proteins:
  • LacR (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry