pAdEasy-Msln-iCre-HA-Flag
(Plasmid #31305)

• Depositing Lab: William Pu
• Publication: Zhou et al J Clin Invest. 2011 May 2. 121(5):1894-904.

Backbone

• Vector backbone: pAdEasy
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 33000
• Vector type: Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mesothelin
• Alt name: Msln
• Species: H. sapiens (human)
• Insert Size (bp): 1850
• Entrez Gene: MSLN (a.k.a. MPF, SMRP)
• Tags / Fusion Proteins:
  • Flag (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CGCGTTTTCATCATTGTC

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Msln Promoter sequence provided by author (see above link)

Please note that Addgene was unable to sequence verify this plasmid. The depositing laboratory PCR amplified two regions of this plasmid using the following primer pairs and sequenced the resulting PCR products to confirm the plasmid.

Msln-1F: TCATTTGTTCCCTTTGACGGC
Msln-1R: CTCTCCCCAGCATCCACATTC
Expect 987 nt product

Msln-2F: TTGCCTACAACACCCTGCTGCG
Msln-2R: GCTCACAATGCTTCCATCAAACG
Expect 792 nt product