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pSG5-P190
(Plasmid #31285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bcr-abl fusion protein P190
  • Alt name
    P190 BCR/ABL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    10000
  • Entrez Gene
    ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
  • Entrez Gene
    BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer t7
  • 3′ sequencing primer SV40-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct encodes a human chimeric P190-encoding Bcr/Abl oncogene.

The construct was made by Leena Haataja in 1993.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-P190 was a gift from Nora Heisterkamp (Addgene plasmid # 31285 ; http://n2t.net/addgene:31285 ; RRID:Addgene_31285)

  • For your References section:

    Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for the small GTPase Rap1. Yi SJ, Lee HT, Groffen J, Heisterkamp N. Int J Mol Med. 2008 Oct . 22(4):453-8. PubMed 18813851
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