pSG5-P190
(Plasmid #31285)

• Depositing Lab: Nora Heisterkamp
• Publication: Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Backbone

• Vector backbone: pSG5
• Backbone size w/o insert (bp): 4076
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bcr-abl fusion protein P190
• Alt name: P190 BCR/ABL
• Species: H. sapiens (human)
• Insert Size (bp): 10000
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: t7
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct encodes a human chimeric P190-encoding Bcr/Abl oncogene.

The construct was made by Leena Haataja in 1993.