Skip to main content
Addgene

pBC2FG
(Plasmid #31281)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31281 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFastBacHTa
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4856
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cry2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1803
  • Entrez Gene
    Cry2 (a.k.a. D130054K12Rik)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBC2FG was a gift from Aziz Sancar (Addgene plasmid # 31281 ; http://n2t.net/addgene:31281 ; RRID:Addgene_31281)

  • For your References section:

    Biochemical analysis of the canonical model for the mammalian circadian clock. Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A. J Biol Chem. 2011 May 27. ():. 10.1074/jbc.M111.254680 PubMed 21613214
Related items:
Commonly requested with: