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Addgene

pCKFB1
(Plasmid #31280)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBacHTa
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4856
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clock
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2595
  • Entrez Gene
    Clock (a.k.a. 5330400M04Rik, KAT13D)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCKFB1 was a gift from Aziz Sancar (Addgene plasmid # 31280 ; http://n2t.net/addgene:31280 ; RRID:Addgene_31280)

  • For your References section:

    Biochemical analysis of the canonical model for the mammalian circadian clock. Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A. J Biol Chem. 2011 May 27. ():. 10.1074/jbc.M111.254680 PubMed 21613214
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