pMAM-RecG-ST2TaV-EGFP
(Plasmid
#31275)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3992
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRecG-ST2-TaV-EGFP
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Alt nameRecG
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Alt nameEGFP
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SpeciesE. coli
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Insert Size (bp)2949
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GenBank IDNC_010473
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer various (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAM-RecG-ST2TaV-EGFP was a gift from Andrew Pierce (Addgene plasmid # 31275 ; http://n2t.net/addgene:31275 ; RRID:Addgene_31275) -
For your References section:
Escherichia coli RecG functionally suppresses human Bloom syndrome phenotypes. Killen MW, Stults DM, Wilson WA, Pierce AJ. BMC Mol Biol. 2012 Oct 30;13:33. doi: 10.1186/1471-2199-13-33. 10.1186/1471-2199-13-33 PubMed 23110454
