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Addgene

pMAM-RecG-EGFP
(Plasmid #31274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3992
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RecG-EGFP
  • Species
    E. coli
  • Insert Size (bp)
    2853
  • GenBank ID
    NC_010473
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer various
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAM-RecG-EGFP was a gift from Andrew Pierce (Addgene plasmid # 31274 ; http://n2t.net/addgene:31274 ; RRID:Addgene_31274)

  • For your References section:

    Escherichia coli RecG functionally suppresses human Bloom syndrome phenotypes. Killen MW, Stults DM, Wilson WA, Pierce AJ. BMC Mol Biol. 2012 Oct 30;13:33. doi: 10.1186/1471-2199-13-33. 10.1186/1471-2199-13-33 PubMed 23110454
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