pmTgfa UTR
(Plasmid #31256)

• Depositing Lab: David Lee
• Publication: Kornblum et al J Comp Neurol. 1997 Apr 7. 380(2):243-61.

Backbone

• Vector backbone: pGEM-3Z
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2800
• Vector type: cDNA for in situ hybridization

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tgfa
• Species: M. musculus (mouse)
• Mutation: Exon 6 of Tgfa with 3'UTR
• Entrez Gene: Tgfa (a.k.a. wa-1, wa1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are 2 nucleotide mismatches with NCBI reference sequence but they will not affect plasmid function as a riboprobe.

For Riboprobe, cut with BamHI. Runoffs with SP6 polymerase.

This construct was used in Assimacopoulos et al, J Neurosci. 2003 (PMID: 12878679).