pcDNA3.1MycHis/HIP1/ΔE
(Plasmid
#31252)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31252 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5440
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuntingtin-interacting protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)3826
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Mutationdeleted ENTH domain
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Entrez GeneHIP1 (a.k.a. HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma)
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Tags
/ Fusion Proteins
- myc (C terminal on backbone)
- his (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1MycHis/HIP1/ΔE was a gift from Theodora Ross (Addgene plasmid # 31252 ; http://n2t.net/addgene:31252 ; RRID:Addgene_31252) -
For your References section:
Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival. Rao DS, Hyun TS, Kumar PD, Mizukami IF, Rubin MA, Lucas PC, Sanda MG, Ross TS. J Clin Invest. 2002 Aug . 110(3):351-60. 10.1172/JCI15529 PubMed 12163454