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Addgene

pcDNA3/HIP1/ΔC
(Plasmid #31250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Huntingtin-interacting protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3246
  • Mutation
    Deltion of nucleotides 1336-2513 1386bp C to T mutation for Bpu10I restriction site
  • Entrez Gene
    HIP1 (a.k.a. HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/HIP1/ΔC was a gift from Theodora Ross (Addgene plasmid # 31250 ; http://n2t.net/addgene:31250 ; RRID:Addgene_31250)

  • For your References section:

    Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival. Rao DS, Hyun TS, Kumar PD, Mizukami IF, Rubin MA, Lucas PC, Sanda MG, Ross TS. J Clin Invest. 2002 Aug . 110(3):351-60. 10.1172/JCI15529 PubMed 12163454
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