pmSfrp2
(Plasmid
#31249)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typecDNA for in situ hybridization
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSfrp2
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SpeciesM. musculus (mouse)
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Entrez GeneSfrp2 (a.k.a. AI851596, Sdf5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For Riboprobe, cut with XbaI. Runoffs with T7 polymerase.
This construct was used in Assimacopoulos et al, J Neurosci. 2003 (PMID:
12878679).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmSfrp2 was a gift from Jeremy Nathans (Addgene plasmid # 31249 ; http://n2t.net/addgene:31249 ; RRID:Addgene_31249) -
For your References section:
A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors. Rattner A, Hsieh JC, Smallwood PM, Gilbert DJ, Copeland NG, Jenkins NA, Nathans J. Proc Natl Acad Sci U S A. 1997 Apr 1. 94(7):2859-63. 10.1073/pnas.94.7.2859 PubMed 9096311