pDM#834
(Plasmid #31240)

• Purpose: (Empty Backbone)
• Depositing Lab: Don Moerman
• Publication: Meissner et al PLoS One. 2011 . 6(5):e19937.

Backbone

• Vector backbone: pPD95.75
• Backbone manufacturer: Andrew Fire (Addgene plasmid# 1494)
• Backbone size (bp): 7980
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Species: C. elegans (nematode)
• Tag / Fusion Protein:
  • GST (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IMPORTANT: Though the map depicts suggests that the CAT/CamR gene is present, this plasmid does NOT confer Chloramphenicol resistance. That gene is a remnant of the cloning and is not functional.

From the depositor: "The clone is a GFP fusion vector that carries a C. elegans muscle specific promoter in front of the GFP sequence as a pDEST destination vector. Protein coding sequences are introduced into this vector between the promoter and GFP sequences by recombination from a pDonor clone using the Invitrogen clonase kit. The backbone vector was pPD95_75 and the promoter and pDEST recombination sequences were cloned into this vector."

Please note that there are discrepancies between the author's sequence and Addgene's quality control sequence, but the depositing lab does not believe these differences should affect function.