pmTUBB3
(Plasmid
#31236)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK(+)
- Backbone size w/o insert (bp) 3000
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Vector typecDNA for in situ hybridization
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTUBB3
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Alt nameclass III B-tubulin
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SpeciesM. musculus (mouse)
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Insert Size (bp)321
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Mutation3'UTR sequence
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Entrez GeneTubb3 (a.k.a. 3200002H15Rik, M(beta)3, M(beta)6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For riboprobes, cut with BamHI. Runoffs with T7.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmTUBB3 was a gift from Cliff Ragsdale (Addgene plasmid # 31236 ; http://n2t.net/addgene:31236 ; RRID:Addgene_31236) -
For your References section:
The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Grove EA, Tole S, Limon J, Yip L, Ragsdale CW. Development. 1998 Jun . 125(12):2315-25. PubMed 9584130