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Addgene

pcDISP1
(Plasmid #31235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3950
  • Vector type
    cDNA for in situ hybridization

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DISP1
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    631
  • Entrez Gene
    DISP1

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (unknown if destroyed)
  • 3′ cloning site TOPO (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDISP1 was a gift from Cliff Ragsdale (Addgene plasmid # 31235 ; http://n2t.net/addgene:31235 ; RRID:Addgene_31235)

  • For your References section:

    Differential susceptibility of midbrain and spinal cord patterning to floor plate defects in the talpid2 mutant. Agarwala S, Aglyamova GV, Marma AK, Fallon JF, Ragsdale CW. Dev Biol. 2005 Dec 1. 288(1):206-20. 10.1016/j.ydbio.2005.09.034 PubMed 16246323
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