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Addgene

pEFX-AP
(Plasmid #31234)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31234 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEFX (modified pEF1/myc-His C)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP
  • Alt name
    alkaline phosphatase
  • Species
    H. sapiens (human)
  • Entrez Gene
    ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pEF1/myc-His C (Invitrogen) was modified to make pEFX by excising a 2.2kb PvuII fragment containing Neomycin and SV40 elements.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFX-AP was a gift from Cliff Ragsdale (Addgene plasmid # 31234 ; http://n2t.net/addgene:31234 ; RRID:Addgene_31234)
  • For your References section:

    Sonic hedgehog control of size and shape in midbrain pattern formation. Agarwala S, Sanders TA, Ragsdale CW. Science. 2001 Mar 16. 291(5511):2147-50. 10.1126/science.1058624 PubMed 11251119