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Addgene

pcTUBB3
(Plasmid #31232)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    cDNA for in situ hybridization

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TUBB3
  • Alt name
    class III B-tubulin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    434
  • Mutation
    C-terminal + 3'UTR sequence
  • Entrez Gene
    TUBB3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstZ1 (unknown if destroyed)
  • 3′ cloning site SmaI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For riboprobes, cut with EagI. Runoffs with T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcTUBB3 was a gift from Cliff Ragsdale (Addgene plasmid # 31232 ; http://n2t.net/addgene:31232 ; RRID:Addgene_31232)

  • For your References section:

    Arcuate plan of chick midbrain development. Sanders TA, Lumsden A, Ragsdale CW. J Neurosci. 2002 Dec 15. 22(24):10742-50. PubMed 12486167
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