pfGAD65
(Plasmid #31231)

• Depositing Lab: Cliff Ragsdale
• Publication: Rowell et al J Comp Neurol. 2010 Aug 15. 518(16):3272-89.

Backbone

• Vector backbone: pCRII-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3950
• Vector type: cDNA for in situ hybridization

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAD2
• Alt name: GAD65
• Species: Mustela putorius furo
• Insert Size (bp): 1015

Cloning Information

• Cloning method: TOPO Cloning
• 5′ cloning site: TOPO (unknown if destroyed)
• 3′ cloning site: TOPO (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For riboprobe cut with EcoRI. Runoffs with SP6.