UbC NLS-HA-MCP-YFP
(Plasmid #31230)

• Depositing Lab: Robert Singer
• Publication: Grunwald et al Nature. 2010 Sep 30. 467(7315):604-7.

Backbone

• Vector backbone: pHAGE
• Backbone size w/o insert (bp): 6247
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NLS-HA-MCP-YFP
• Species: artificial
• Insert Size (bp): 1171

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The phage-UbC RIG vector for lentiviral expression was a gift from G. Mostoslavsky and G. Vainer (Harvard University). Mostoslavsky, G., Fabian, A.J., Rooney, S., Alt, F.W. & Mulligan, R.C. Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer. Proc. Natl. Acad. Sci. USA 103, 16406–16411 (2006).
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional reference: Lionnet T, Czaplinski K, Darzacq X, Shav-Tal Y, Wells AL, Chao JA, Park HY, de Turris V, Lopez-Jones M, Singer RH. A transgenic mouse for in vivo detection of endogenous labeled mRNA. Nat Methods. Feb;8(2):165-70 (2011)