pPAGFP-TPX2
(Plasmid #31226)

• Depositing Lab: Patricia Wadsworth
• Publication: Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88.

Backbone

• Vector backbone: pPAGFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TPX2
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Entrez Gene: TPX2 (a.k.a. C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100)
• Tag / Fusion Protein:
  • PAGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspEI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pPAGFP-C1 plasmid was from Drs. G Patterson and J.Lippincott-Schwartz
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry