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Addgene

pKEB31
(Plasmid #31224)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDD62
  • Backbone size w/o insert (bp) 12617

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    ccdB survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ccdB and Cm resistance gene
  • Insert Size (bp)
    1447

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CAT-R (for 1st attR)
  • 3′ sequencing primer Tet-R (for 2nd attR)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKEB31 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31224 ; http://n2t.net/addgene:31224 ; RRID:Addgene_31224)

  • For your References section:

    Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218 10.1093/nar/gkr218 PubMed 21493687
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