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pLenti6/V5-DEST-SPAG5
(Plasmid #31217)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31217 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPAG5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3582
  • Mutation
    Contains a one nucleotide deletion at C-term that shifts out-of-frame with V5 tag in pLent6 V5/DEST; alters last four residues (EFLS* >LNF*)
  • GenBank ID
    BC000322
  • Entrez Gene
    SPAG5 (a.k.a. DEEPEST, MAP126, hMAP126)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (destroyed during cloning)
  • 3′ cloning site attR2 (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer V5 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6/V5-DEST-SPAG5 was a gift from Lynda Chin (Addgene plasmid # 31217 ; http://n2t.net/addgene:31217 ; RRID:Addgene_31217)

  • For your References section:

    Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA, Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, DePinho RA, Rimm DL, Chin L. Cancer Cell. 2011 Jul 12;20(1):92-103. 10.1016/j.ccr.2011.05.025 PubMed 21741599
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