pLenti6/V5-DEST-MCM7
(Plasmid #31212)

• Depositing Lab: Lynda Chin
• Publication: Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Backbone

• Vector backbone: pLenti6/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCM7
• Species: H. sapiens (human)
• Insert Size (bp): 2160
• GenBank ID: BC013375
• Entrez Gene: MCM7 (a.k.a. CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: V5 Reverse

Resource Information

• Addgene Notes:
  • Feedback from Mr. Bastian Gastl; Charité Berlin - Institute of Pathology
• A portion of this plasmid was derived from a plasmid made by: The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry