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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Growth instructionsGrow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFASCIN
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SpeciesH. sapiens (human)
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Insert Size (bp)1482
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GenBank IDBC000521
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Entrez GeneFSCN1 (a.k.a. FAN1, HSN, SNL, p55)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer V5 Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6/V5-DEST-FASCIN was a gift from Lynda Chin (Addgene plasmid # 31207 ; http://n2t.net/addgene:31207 ; RRID:Addgene_31207) -
For your References section:
Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA, Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, DePinho RA, Rimm DL, Chin L. Cancer Cell. 2011 Jul 12;20(1):92-103. 10.1016/j.ccr.2011.05.025 PubMed 21741599